147 lines
11 KiB
HTML
147 lines
11 KiB
HTML
<!-- SOP-CC-006-E01-01 集落培养操作记录(A4 竖版) -->
|
|
<div class="sop-record sop-a4 sop-cc-006" style="font-family: 'PingFang SC','Microsoft YaHei',sans-serif; font-size: 10.5pt; color: #1e293b; line-height: 1.55; box-sizing: border-box; display: flex; flex-direction: column; height: 297mm; min-height: 297mm; padding: 16mm 15mm 20mm;">
|
|
<table class="sop-header-table sop-row-hidden" style="width:100%; border-collapse:collapse; table-layout:fixed; border:1px solid #334155; margin-bottom:8px; flex-shrink:0;">
|
|
<colgroup>
|
|
<col style="width:15%" />
|
|
<col style="width:43%" />
|
|
<col style="width:42%" />
|
|
</colgroup>
|
|
<tbody>
|
|
<tr>
|
|
<td rowspan="2" style="border:1px solid #334155; padding:6px; text-align:center; vertical-align:middle;">
|
|
<img src="/sop-templates/SOP-CC-006-logo.jpeg" alt="logo" width="48" height="48" style="display:block; margin:0 auto;" />
|
|
</td>
|
|
<td rowspan="2" style="border:1px solid #334155; padding:8px; text-align:center; vertical-align:middle; font-family: SimSun, 'Songti SC', serif; font-size: 12pt; font-weight:700;">
|
|
集落培养操作记录
|
|
</td>
|
|
<td style="border:1px solid #334155; padding:6px 10px; font-size: 12pt; vertical-align:middle;">
|
|
文件编码: SOP-CC-006-E01
|
|
</td>
|
|
</tr>
|
|
<tr>
|
|
<td style="border:1px solid #334155; padding:6px 10px; font-size: 12pt; vertical-align:middle;">
|
|
版 本 号: 01
|
|
</td>
|
|
</tr>
|
|
</tbody>
|
|
</table>
|
|
|
|
<table class="sop-body-table" style="width:100%; border-collapse:collapse; table-layout:fixed; border:1px solid #334155; flex:1 1 auto; height:100%; min-height:0;">
|
|
<colgroup>
|
|
<col style="width:8.6%" />
|
|
<col style="width:2.7%" />
|
|
<col style="width:20.2%" />
|
|
<col style="width:1.4%" />
|
|
<col style="width:2.4%" />
|
|
<col style="width:14.7%" />
|
|
<col style="width:5.7%" />
|
|
<col style="width:5.7%" />
|
|
<col style="width:2.4%" />
|
|
<col style="width:17.1%" />
|
|
<col style="width:5.2%" />
|
|
<col style="width:13.9%" />
|
|
</colgroup>
|
|
<tbody><tr class="sop-row-hidden sop-row-hidden">
|
|
<td colspan="6" style="border:1px solid #334155; padding:8px;">工序: 集落培养操作记录</td>
|
|
<td colspan="2" style="border:1px solid #334155; padding:8px; white-space:nowrap;">记录编号</td>
|
|
<td colspan="4" class="sop-blank-cell" style="border:1px solid #334155; padding:8px; min-height:28px;"> </td>
|
|
</tr><tr class="sop-row-hidden sop-row-hidden">
|
|
<td colspan="2" style="border:1px solid #334155; padding:8px; white-space:nowrap;">细胞批号</td>
|
|
<td colspan="4" class="sop-blank-cell" style="border:1px solid #334155; padding:8px;"> </td>
|
|
<td colspan="2" style="border:1px solid #334155; padding:8px; white-space:nowrap;">日期</td>
|
|
<td colspan="4" class="sop-blank-cell" style="border:1px solid #334155; padding:8px;"> </td>
|
|
</tr><tr class="sop-row-hidden sop-row-hidden">
|
|
<td rowspan="4" style="border:1px solid #334155; padding:8px; text-align:center; vertical-align:middle;">试剂</td>
|
|
<td colspan="2" style="border:1px solid #334155; padding:8px; text-align:center;">名称</td>
|
|
<td colspan="5" style="border:1px solid #334155; padding:8px; text-align:center;">生产厂家</td>
|
|
<td colspan="2" style="border:1px solid #334155; padding:8px; text-align:center;">批号</td>
|
|
<td colspan="2" style="border:1px solid #334155; padding:8px; text-align:center;">使用数量</td>
|
|
</tr><tr class="sop-row-hidden sop-row-hidden">
|
|
<td colspan="2" style="border:1px solid #334155; padding:8px;">CFU-GM试剂</td>
|
|
<td colspan="5" class="sop-blank-cell" style="border:1px solid #334155; padding:8px;"> </td>
|
|
<td colspan="2" class="sop-blank-cell" style="border:1px solid #334155; padding:8px;"> </td>
|
|
<td colspan="2" class="sop-blank-cell" style="border:1px solid #334155; padding:8px;"> </td>
|
|
</tr><tr class="sop-row-hidden sop-row-hidden">
|
|
<td colspan="2" style="border:1px solid #334155; padding:8px;">HES试剂</td>
|
|
<td colspan="5" class="sop-blank-cell" style="border:1px solid #334155; padding:8px;"> </td>
|
|
<td colspan="2" class="sop-blank-cell" style="border:1px solid #334155; padding:8px;"> </td>
|
|
<td colspan="2" class="sop-blank-cell" style="border:1px solid #334155; padding:8px;"> </td>
|
|
</tr><tr class="sop-row-hidden sop-row-hidden">
|
|
<td colspan="2" style="border:1px solid #334155; padding:8px;">低糖DMEM培养基</td>
|
|
<td colspan="5" class="sop-blank-cell" style="border:1px solid #334155; padding:8px;"> </td>
|
|
<td colspan="2" class="sop-blank-cell" style="border:1px solid #334155; padding:8px;"> </td>
|
|
<td colspan="2" class="sop-blank-cell" style="border:1px solid #334155; padding:8px;"> </td>
|
|
</tr><tr class="sop-row-hidden sop-row-hidden">
|
|
<td style="border:1px solid #334155; padding:8px; text-align:center; vertical-align:middle;">仪器<br />设备</td>
|
|
<td colspan="11" style="border:1px solid #334155; padding:8px; vertical-align:top;">
|
|
<div>
|
|
操作台:
|
|
<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>XW022</span>
|
|
<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>XW037</span>
|
|
<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>其他</span>
|
|
<span class="sop-underline" style="text-align:left; min-width:80px;"> </span>
|
|
</div>
|
|
<div style="margin-top:6px;">
|
|
流式细胞仪:
|
|
<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>XW042</span>
|
|
<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>其他</span>
|
|
<span class="sop-underline" style="text-align:left; min-width:80px;"> </span>
|
|
</div>
|
|
</td>
|
|
</tr><tr class="sop-fill-row">
|
|
<td class="sop-fill-label" style="border:1px solid #334155; padding:8px; text-align:center; vertical-align:middle; height:100%;">造血干细胞<br />集落培养<br />操作记录</td>
|
|
<td class="sop-fill-cell" colspan="11" style="border:1px solid #334155; padding:10px; vertical-align:top; height:100%;">
|
|
<p style="margin:0 0 6px;">
|
|
1. 向1号EP管中加入血样<span class="sop-underline" style="text-align:left; min-width:56px;"> </span>μL、DMEM培养基<span class="sop-underline" style="text-align:left; min-width:56px;"> </span>μL, HES试剂<span class="sop-underline" style="text-align:left; min-width:56px;"> </span>μL, 混匀后静置沉降20min.
|
|
</p>
|
|
<p style="margin:0 0 6px;">
|
|
2. 吸取CFU-GM试剂<span class="sop-underline" style="text-align:left; min-width:56px;"> </span>μL, 加入48孔板, 放入培养箱中静置10min.
|
|
</p>
|
|
<p style="margin:0 0 6px;">3. 吸取1号EP管中血液上清, 加入2号EP管中.</p>
|
|
<p style="margin:0 0 6px;">4. 从2号EP管中吸取50μL, 加入3号EP管中.</p>
|
|
<p style="margin:0 0 6px;">
|
|
5. 拿2号EP管进行五分仪计数, 根据计数结果计算加入3号EP管DMEM培养基<span class="sop-underline" style="text-align:left; min-width:56px;"> </span>μL.
|
|
</p>
|
|
<p style="margin:0;">6. 3号EP管混匀后吸取20μL加入48孔板.</p>
|
|
</td>
|
|
</tr><tr class="sop-row-hidden sop-row-hidden">
|
|
<td colspan="7" style="border:1px solid #334155; padding:8px;">48孔板后续操作</td>
|
|
<td colspan="5" class="sop-blank-cell" style="border:1px solid #334155; padding:8px;"> </td>
|
|
</tr><tr class="sop-row-hidden sop-row-hidden">
|
|
<td colspan="7" style="border:1px solid #334155; padding:8px;">结果信息</td>
|
|
<td colspan="5" class="sop-blank-cell" style="border:1px solid #334155; padding:8px;"> </td>
|
|
</tr><tr class="sop-row-hidden sop-row-hidden">
|
|
<td rowspan="3" style="border:1px solid #334155; padding:8px; text-align:center; vertical-align:middle;">清场</td>
|
|
<td colspan="6" style="border:1px solid #334155; padding:8px;">未使用完试剂是否放入指定位置</td>
|
|
<td colspan="5" class="sop-option-cell" style="border:1px solid #334155; padding:8px; text-align:center;">
|
|
<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>是</span>
|
|
<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>否</span>
|
|
</td>
|
|
</tr><tr class="sop-row-hidden sop-row-hidden">
|
|
<td colspan="6" style="border:1px solid #334155; padding:8px;">废液是否进行收集处理</td>
|
|
<td colspan="5" class="sop-option-cell" style="border:1px solid #334155; padding:8px; text-align:center;">
|
|
<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>是</span>
|
|
<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>否</span>
|
|
</td>
|
|
</tr><tr class="sop-row-hidden sop-row-hidden">
|
|
<td colspan="4" style="border:1px solid #334155; padding:8px; white-space:nowrap;">是否清洁, 消毒操作间</td>
|
|
<td colspan="1" class="sop-option-cell" style="border:1px solid #334155; padding:8px; white-space:nowrap; text-align:center;">
|
|
<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>是</span>
|
|
<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>否</span>
|
|
</td>
|
|
<td colspan="5" style="border:1px solid #334155; padding:8px; white-space:nowrap;">是否清洁, 消毒操作台与仪器</td>
|
|
<td colspan="1" class="sop-option-cell" style="border:1px solid #334155; padding:8px; white-space:nowrap; text-align:center;">
|
|
<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>是</span>
|
|
<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>否</span>
|
|
</td>
|
|
</tr><tr class="sop-row-hidden sop-row-hidden">
|
|
<td style="border:1px solid #334155; padding:8px; text-align:center;">备注</td>
|
|
<td colspan="11" style="border:1px solid #334155; padding:8px; min-height:48px; height:48px; vertical-align:top;"> </td>
|
|
</tr><tr class="sop-row-hidden sop-row-hidden">
|
|
<td colspan="4" style="border:1px solid #334155; padding:10px;">操作人/日期:</td>
|
|
<td colspan="5" style="border:1px solid #334155; padding:10px;">复核人/日期:</td>
|
|
<td colspan="3" style="border:1px solid #334155; padding:10px;">主管/日期:</td>
|
|
</tr></tbody>
|
|
</table>
|
|
</div>
|