206 lines
16 KiB
SQL
206 lines
16 KiB
SQL
-- 种子数据: SOP-SC-004-E01-01 SOP-SC-004-E01-01 人脐带间充质干细胞原代培养半换液操作记录
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-- 源文件: docs/sop-templates/SOP-SC-004-E01-01.html
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UPDATE `biz_process`
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SET `sop_content` = '<!-- SOP-SC-004-E01-01 人脐带间充质干细胞原代培养半换液操作记录(A4 竖版) -->
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<div class="sop-record sop-a4 sop-sc-004" style="font-family: ''PingFang SC'',''Microsoft YaHei'',sans-serif; font-size: 10.5pt; color: #1e293b; line-height: 1.55; box-sizing: border-box; display: flex; flex-direction: column; height: 297mm; min-height: 297mm; padding: 16mm 15mm 20mm;">
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<table class="sop-header-table sop-row-hidden" style="width:100%; border-collapse:collapse; table-layout:fixed; border:1px solid #334155; margin-bottom:8px; flex-shrink:0;">
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<colgroup>
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<col style="width:15%" />
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<col style="width:50%" />
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<col style="width:35%" />
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</colgroup>
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<tbody>
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<tr>
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<td rowspan="2" style="border:1px solid #334155; padding:6px; text-align:center; vertical-align:middle;">
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<img src="/sop-templates/SOP-SC-004-logo.jpeg" alt="logo" width="48" height="48" style="display:block; margin:0 auto;" />
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</td>
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<td rowspan="2" style="border:1px solid #334155; padding:8px; text-align:center; vertical-align:middle; font-family: SimSun, ''Songti SC'', serif; font-size: 11pt; font-weight:700;">
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人脐带间充质干细胞原代培养半换液操作记录
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</td>
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<td style="border:1px solid #334155; padding:6px 10px; font-size: 12pt; vertical-align:middle;">
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文件编码: SOP-SC-004-E01
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</td>
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</tr>
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<tr>
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<td style="border:1px solid #334155; padding:6px 10px; font-size: 12pt; vertical-align:middle;">
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版 本 号: 01
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</td>
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</tr>
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</tbody>
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</table>
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<table class="sop-body-table" style="width:100%; border-collapse:collapse; table-layout:fixed; border:1px solid #334155; flex:1 1 auto; height:100%; min-height:0;">
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<colgroup>
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<col style="width:7.0%" />
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<col style="width:4.4%" />
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<col style="width:20.3%" />
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<col style="width:1.4%" />
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<col style="width:2.4%" />
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<col style="width:14.7%" />
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<col style="width:5.8%" />
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<col style="width:5.6%" />
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<col style="width:2.5%" />
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<col style="width:17.1%" />
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<col style="width:5.2%" />
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<col style="width:13.6%" />
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</colgroup>
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<tbody><tr class="sop-row-hidden sop-row-hidden">
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<td colspan="6" style="border:1px solid #334155; padding:8px;">工序: 脐带MSC原代细胞培养半换液</td>
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<td colspan="2" style="border:1px solid #334155; padding:8px; white-space:nowrap;">记录编号</td>
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<td colspan="4" class="sop-blank-cell" style="border:1px solid #334155; padding:8px; min-height:28px;"> </td>
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</tr><tr class="sop-row-hidden sop-row-hidden">
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<td colspan="2" style="border:1px solid #334155; padding:8px; white-space:nowrap;">细胞批号</td>
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<td colspan="4" class="sop-blank-cell" style="border:1px solid #334155; padding:8px;"> </td>
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<td colspan="2" style="border:1px solid #334155; padding:8px; white-space:nowrap;">日期</td>
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<td colspan="4" class="sop-blank-cell" style="border:1px solid #334155; padding:8px;"> </td>
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</tr><tr class="sop-row-hidden sop-row-hidden">
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<td rowspan="2" style="border:1px solid #334155; padding:8px; text-align:center; vertical-align:middle;">试剂</td>
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<td colspan="2" style="border:1px solid #334155; padding:8px; text-align:center;">名称</td>
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<td colspan="5" style="border:1px solid #334155; padding:8px; text-align:center;">生产厂家</td>
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<td colspan="2" style="border:1px solid #334155; padding:8px; text-align:center;">批号</td>
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<td colspan="2" style="border:1px solid #334155; padding:8px; text-align:center;">使用数量</td>
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</tr><tr class="sop-row-hidden sop-row-hidden">
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<td colspan="2" style="border:1px solid #334155; padding:8px;">干细胞培养基</td>
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<td colspan="5" class="sop-blank-cell" style="border:1px solid #334155; padding:8px;"> </td>
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<td colspan="2" class="sop-blank-cell" style="border:1px solid #334155; padding:8px;"> </td>
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<td colspan="2" class="sop-blank-cell" style="border:1px solid #334155; padding:8px;"> </td>
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</tr><tr class="sop-row-hidden sop-row-hidden">
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<td style="border:1px solid #334155; padding:8px; text-align:center; vertical-align:middle;">仪器<br />设备</td>
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<td colspan="11" style="border:1px solid #334155; padding:8px; vertical-align:top;">
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<div>
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生物安全柜:
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<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>XW015</span>
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<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>XW022</span>
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<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>其他</span>
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<span class="sop-underline" style="text-align:left; min-width:80px;"> </span>
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</div>
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<div style="margin-top:6px;">
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离心机:
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<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>XW015</span>
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<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>XW022</span>
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<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>其他</span>
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<span class="sop-underline" style="text-align:left; min-width:80px;"> </span>
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</div>
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</td>
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</tr><tr class="sop-fill-row">
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<td class="sop-fill-label" style="border:1px solid #334155; padding:8px; text-align:center; vertical-align:middle; height:100%;">干细胞原代<br />培养半换液</td>
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<td class="sop-fill-cell" colspan="11" style="border:1px solid #334155; padding:10px; vertical-align:top; height:100%;">
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<p style="margin:0 0 6px;">
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1. 原代培养48h内, 观察细胞
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<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>已经</span>
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<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>还未</span>
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爬出, 进行补液;
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</p>
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<p style="margin:0 0 6px;">
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2. 在培养瓶表面喷洒酒精后, 用无尘布擦拭放入生物安全柜中, 打开瓶盖, 使用10mL移液管轻轻加入
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<span class="sop-underline" style="text-align:left; min-width:56px;"> </span>mL, 将体系补至30mL;
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</p>
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<p style="margin:0 0 6px;">3. 静置细胞7天;</p>
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<p style="margin:0 0 6px;">
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4. Day<span class="sop-underline" style="text-align:left; min-width:40px;"> </span>观察细胞
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<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>已经</span>
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<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>还未</span>
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爬出, 进行半换液;
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</p>
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<p style="margin:0 0 6px;">
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5. 在培养瓶表面喷洒酒精后, 用无尘布擦拭放入生物安全柜中, 打开瓶盖, 使用10mL移液管轻轻吸弃上清
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<span class="sop-underline" style="text-align:left; min-width:56px;"> </span>mL, 再加入等量体积的MSC完全培养基;
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</p>
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<p style="margin:0 0 6px;">
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6. Day<span class="sop-underline" style="text-align:left; min-width:40px;"> </span>观察细胞
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<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>已经</span>
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<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>还未</span>
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爬出, 进行半换液;
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</p>
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<p style="margin:0 0 6px;">
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7. 在培养瓶表面喷洒酒精后, 用无尘布擦拭放入生物安全柜中, 打开瓶盖, 使用10mL移液管轻轻吸弃上清
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<span class="sop-underline" style="text-align:left; min-width:56px;"> </span>mL, 再加入等量体积的MSC完全培养基;
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</p>
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<p style="margin:0 0 6px;">
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8. Day<span class="sop-underline" style="text-align:left; min-width:40px;"> </span>观察细胞
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<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>已经</span>
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<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>还未</span>
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爬出, 进行半换液;
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</p>
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<p style="margin:0 0 6px;">
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9. 在培养瓶表面喷洒酒精后, 用无尘布擦拭放入生物安全柜中, 打开瓶盖, 使用10mL移液管轻轻吸弃上清
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<span class="sop-underline" style="text-align:left; min-width:56px;"> </span>mL, 再加入等量体积的MSC完全培养基;
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</p>
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<p style="margin:0 0 6px;">
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10. Day<span class="sop-underline" style="text-align:left; min-width:40px;"> </span>观察细胞
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<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>已经</span>
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<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>还未</span>
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爬出, 进行半换液;
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</p>
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<p style="margin:0 0 6px;">
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11. 在培养瓶表面喷洒酒精后, 用无尘布擦拭放入生物安全柜中, 打开瓶盖, 使用10mL移液管轻轻吸弃上清
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<span class="sop-underline" style="text-align:left; min-width:56px;"> </span>mL, 再加入等量体积的MSC完全培养基;
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</p>
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<p style="margin:0 0 6px;">
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12. Day<span class="sop-underline" style="text-align:left; min-width:40px;"> </span>观察细胞
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<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>已经</span>
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<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>还未</span>
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爬出, 进行半换液;
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</p>
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<p style="margin:0 0 6px;">
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13. 在培养瓶表面喷洒酒精后, 用无尘布擦拭放入生物安全柜中, 打开瓶盖, 使用10mL移液管轻轻吸弃上清
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<span class="sop-underline" style="text-align:left; min-width:56px;"> </span>mL, 再加入等量体积的MSC完全培养基;
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</p>
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<p style="margin:0;">
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14. Day<span class="sop-underline" style="text-align:left; min-width:40px;"> </span>观察到爬出的细胞局部融合度达到80%-90%, 准备进行重铺.
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</p>
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</td>
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</tr><tr class="sop-row-hidden sop-row-hidden">
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<td style="border:1px solid #334155; padding:8px; text-align:center; vertical-align:middle;">小结</td>
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<td colspan="11" style="border:1px solid #334155; padding:8px; vertical-align:top;">
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细胞于Day <span class="sop-underline" style="text-align:left; min-width:40px;"> </span>开始观察到爬出; Day<span class="sop-underline" style="text-align:left; min-width:40px;"> </span>进行重铺,
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<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>是</span>
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<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>否</span>
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进行二次贴壁.
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</td>
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</tr><tr class="sop-row-hidden sop-row-hidden">
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<td colspan="7" style="border:1px solid #334155; padding:8px;">每个培养瓶上是否皆清楚标示细胞批号</td>
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<td colspan="5" class="sop-option-cell" style="border:1px solid #334155; padding:8px; text-align:center;">
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<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>是</span>
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<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>否</span>
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</td>
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</tr><tr class="sop-row-hidden sop-row-hidden">
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<td rowspan="3" style="border:1px solid #334155; padding:8px; text-align:center; vertical-align:middle;">清场</td>
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<td colspan="6" style="border:1px solid #334155; padding:8px;">未使用完试剂是否放入指定位置</td>
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<td colspan="5" class="sop-option-cell" style="border:1px solid #334155; padding:8px; text-align:center;">
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<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>是</span>
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<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>否</span>
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</td>
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</tr><tr class="sop-row-hidden sop-row-hidden">
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<td colspan="6" style="border:1px solid #334155; padding:8px;">废液是否进行收集处理</td>
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<td colspan="5" class="sop-option-cell" style="border:1px solid #334155; padding:8px; text-align:center;">
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<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>是</span>
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<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>否</span>
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</td>
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</tr><tr class="sop-row-hidden sop-row-hidden">
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<td colspan="4" style="border:1px solid #334155; padding:8px; white-space:nowrap;">是否清洁, 消毒操作间</td>
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<td colspan="1" class="sop-option-cell" style="border:1px solid #334155; padding:8px; white-space:nowrap; text-align:center;">
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<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>是</span>
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<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>否</span>
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</td>
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<td colspan="5" style="border:1px solid #334155; padding:8px; white-space:nowrap;">是否清洁, 消毒生物安全柜与移液枪</td>
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<td colspan="1" class="sop-option-cell" style="border:1px solid #334155; padding:8px; white-space:nowrap; text-align:center;">
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<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>是</span>
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<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>否</span>
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</td>
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</tr><tr class="sop-row-hidden sop-row-hidden">
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<td style="border:1px solid #334155; padding:8px; text-align:center;">备注</td>
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<td colspan="11" style="border:1px solid #334155; padding:8px; min-height:48px; height:48px; vertical-align:top;"> </td>
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</tr><tr class="sop-row-hidden sop-row-hidden">
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<td colspan="4" style="border:1px solid #334155; padding:10px;">操作人/日期:</td>
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<td colspan="5" style="border:1px solid #334155; padding:10px;">复核人/日期:</td>
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<td colspan="3" style="border:1px solid #334155; padding:10px;">生产主管/日期:</td>
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</tr></tbody>
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</table>
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</div>
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',
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`update_time` = NOW()
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WHERE `process_code` = 'SOP-SC-004-E01-01'
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AND `del_flag` = 0;
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