180 lines
14 KiB
SQL
180 lines
14 KiB
SQL
-- 种子数据: SOP-SC-003-E01-01 SOP-SC-003-E01-01 人脐带间充质干细胞原代培养操作记录
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-- 源文件: docs/sop-templates/SOP-SC-003-E01-01.html
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UPDATE `biz_process`
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SET `sop_content` = '<!-- SOP-SC-003-E01-01 人脐带间充质干细胞原代培养操作记录(A4 竖版) -->
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<div class="sop-record sop-a4 sop-sc-003" style="font-family: ''PingFang SC'',''Microsoft YaHei'',sans-serif; font-size: 10.5pt; color: #1e293b; line-height: 1.55; box-sizing: border-box; display: flex; flex-direction: column; height: 297mm; min-height: 297mm; padding: 16mm 15mm 20mm;">
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<table class="sop-header-table sop-row-hidden" style="width:100%; border-collapse:collapse; table-layout:fixed; border:1px solid #334155; margin-bottom:8px; flex-shrink:0;">
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<colgroup>
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<col style="width:15%" />
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<col style="width:43%" />
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<col style="width:42%" />
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</colgroup>
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<tbody>
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<tr>
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<td rowspan="2" style="border:1px solid #334155; padding:6px; text-align:center; vertical-align:middle;">
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<img src="/sop-templates/SOP-SC-003-logo.jpeg" alt="logo" width="48" height="48" style="display:block; margin:0 auto;" />
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</td>
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<td rowspan="2" style="border:1px solid #334155; padding:8px; text-align:center; vertical-align:middle; font-family: SimSun, ''Songti SC'', serif; font-size: 12pt; font-weight:700;">
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人脐带间充质干细胞原代培养操作记录
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</td>
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<td style="border:1px solid #334155; padding:6px 10px; font-size: 12pt; vertical-align:middle;">
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文件编码: SOP-SC-003-E01
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</td>
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</tr>
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<tr>
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<td style="border:1px solid #334155; padding:6px 10px; font-size: 12pt; vertical-align:middle;">
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版 本 号: 01
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</td>
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</tr>
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</tbody>
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</table>
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<table class="sop-body-table" style="width:100%; border-collapse:collapse; table-layout:fixed; border:1px solid #334155; flex:1 1 auto; height:100%; min-height:0;">
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<colgroup>
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<col style="width:8.0%" />
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<col style="width:3.3%" />
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<col style="width:20.2%" />
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<col style="width:1.4%" />
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<col style="width:2.4%" />
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<col style="width:14.6%" />
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<col style="width:5.8%" />
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<col style="width:5.6%" />
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<col style="width:2.5%" />
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<col style="width:17.1%" />
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<col style="width:5.1%" />
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<col style="width:14.0%" />
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</colgroup>
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<tbody><tr class="sop-row-hidden sop-row-hidden">
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<td colspan="6" style="border:1px solid #334155; padding:8px;">工序: 脐带MSC原代细胞培养</td>
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<td colspan="2" style="border:1px solid #334155; padding:8px; white-space:nowrap;">记录编号</td>
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<td colspan="4" class="sop-blank-cell" style="border:1px solid #334155; padding:8px; min-height:28px;"> </td>
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</tr><tr class="sop-row-hidden sop-row-hidden">
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<td colspan="2" style="border:1px solid #334155; padding:8px; white-space:nowrap;">细胞批号</td>
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<td colspan="4" class="sop-blank-cell" style="border:1px solid #334155; padding:8px;"> </td>
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<td colspan="2" style="border:1px solid #334155; padding:8px; white-space:nowrap;">日期</td>
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<td colspan="4" class="sop-blank-cell" style="border:1px solid #334155; padding:8px;"> </td>
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</tr><tr class="sop-row-hidden sop-row-hidden">
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<td rowspan="5" style="border:1px solid #334155; padding:8px; text-align:center; vertical-align:middle;">试剂</td>
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<td colspan="2" style="border:1px solid #334155; padding:8px; text-align:center;">名称</td>
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<td colspan="5" style="border:1px solid #334155; padding:8px; text-align:center;">生产厂家</td>
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<td colspan="2" style="border:1px solid #334155; padding:8px; text-align:center;">批号</td>
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<td colspan="2" style="border:1px solid #334155; padding:8px; text-align:center;">使用数量</td>
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</tr><tr class="sop-row-hidden sop-row-hidden">
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<td colspan="2" style="border:1px solid #334155; padding:8px;">磷酸盐缓冲液(PBS)</td>
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<td colspan="5" class="sop-blank-cell" style="border:1px solid #334155; padding:8px;"> </td>
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<td colspan="2" class="sop-blank-cell" style="border:1px solid #334155; padding:8px;"> </td>
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<td colspan="2" class="sop-blank-cell" style="border:1px solid #334155; padding:8px;"> </td>
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</tr><tr class="sop-row-hidden sop-row-hidden">
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<td colspan="2" style="border:1px solid #334155; padding:8px;">MSC培养基</td>
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<td colspan="5" class="sop-blank-cell" style="border:1px solid #334155; padding:8px;"> </td>
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<td colspan="2" class="sop-blank-cell" style="border:1px solid #334155; padding:8px;"> </td>
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<td colspan="2" class="sop-blank-cell" style="border:1px solid #334155; padding:8px;"> </td>
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</tr><tr class="sop-row-hidden sop-row-hidden">
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<td colspan="2" style="border:1px solid #334155; padding:8px;">青/链霉素(双抗)</td>
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<td colspan="5" class="sop-blank-cell" style="border:1px solid #334155; padding:8px;"> </td>
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<td colspan="2" class="sop-blank-cell" style="border:1px solid #334155; padding:8px;"> </td>
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<td colspan="2" class="sop-blank-cell" style="border:1px solid #334155; padding:8px;"> </td>
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</tr><tr class="sop-row-hidden sop-row-hidden">
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<td colspan="2" style="border:1px solid #334155; padding:8px;">75%酒精</td>
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<td colspan="5" class="sop-blank-cell" style="border:1px solid #334155; padding:8px;"> </td>
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<td colspan="2" class="sop-blank-cell" style="border:1px solid #334155; padding:8px;"> </td>
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<td colspan="2" class="sop-blank-cell" style="border:1px solid #334155; padding:8px;"> </td>
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</tr><tr class="sop-row-hidden sop-row-hidden">
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<td style="border:1px solid #334155; padding:8px; text-align:center; vertical-align:middle;">仪器<br />设备</td>
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<td colspan="11" style="border:1px solid #334155; padding:8px; vertical-align:top;">
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<div>
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生物安全柜:
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<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>XW015</span>
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<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>XW022</span>
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<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>其他</span>
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<span class="sop-underline" style="text-align:left; min-width:80px;"> </span>
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</div>
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<div style="margin-top:6px;">
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离心机:
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<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>XW016</span>
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<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>XW026</span>
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<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>其他</span>
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<span class="sop-underline" style="text-align:left; min-width:80px;"> </span>
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</div>
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</td>
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</tr><tr class="sop-fill-row">
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<td class="sop-fill-label" style="border:1px solid #334155; padding:8px; text-align:center; vertical-align:middle; height:100%;">脐带组织处理<br />及干细胞<br />原代培养</td>
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<td class="sop-fill-cell" colspan="11" style="border:1px solid #334155; padding:10px; vertical-align:top; height:100%;">
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<p style="margin:0 0 6px;">
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1. 组织来源: 接收的脐带物料编码<span class="sop-underline" style="text-align:left; min-width:140px;"> </span>. 采集时间:
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<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>24h内</span>
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<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>超过24h</span>;
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入库编码: <span class="sop-underline" style="text-align:left; min-width:120px;"> </span>;
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</p>
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<p style="margin:0 0 6px;">2. 将脐带组织转移至不锈钢碗中;</p>
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<p style="margin:0 0 6px;">
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3. 手术剪裁断脐带, 弃掉结扎部分, 截下一小段留样, 其余部分用清洗液漂洗
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<span class="sop-underline" style="text-align:left; min-width:40px;"> </span>次;
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</p>
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<p style="margin:0 0 6px;">4. 取采集瓶中保存液10mL存于4℃冰箱留样, 留样标签应注明脐带条码, 样本类型, 操作人, 操作日期等;</p>
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<p style="margin:0 0 6px;">
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5. 将脐带在不锈钢碗内, 用清洗液漂洗
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<span class="sop-underline" style="text-align:left; min-width:40px;"> </span>次, 清洗液无明显血色后, 转入培养皿;
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</p>
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<p style="margin:0 0 6px;">
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6. 用手术剪将脐带分成2~3cm的小段, 共计
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<span class="sop-underline" style="text-align:left; min-width:40px;"> </span>段, 放入装有75%酒精的250mL离心瓶中浸泡30s后用清洗液冲洗干净.
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</p>
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<p style="margin:0 0 6px;">7. 用组织镊将脐动脉和脐静脉内的血丝清理掉并洗净;</p>
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<p style="margin:0 0 6px;">8. 由各段脐带组织剥取华通氏胶, 将剥好的华通氏胶放入一个盛有生理盐水的新培养皿内;</p>
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<p style="margin:0 0 6px;">9. 用手术剪将华通氏胶剪成2~4mm<sup>3</sup>大小的组织块, 将剪碎的组织块使用清洗液重悬并200G*5min离心, 取上清存于4℃冰箱待检, 样本标签应注明脐带条码, 样本类型, 操作人, 操作日期等信息;</p>
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<p style="margin:0 0 6px;">
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10. 离心后的组织沉淀共
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<span class="sop-underline" style="text-align:left; min-width:48px;"> </span>mL, 接种
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<span class="sop-underline" style="text-align:left; min-width:40px;"> </span>个T175, 每个T175培养瓶接种
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<span class="sop-underline" style="text-align:left; min-width:48px;"> </span>mL, 接种完成后补加
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<span class="sop-underline" style="text-align:left; min-width:48px;"> </span>mL原代细胞培养液.
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</p>
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<p style="margin:0;">11. 培养瓶上标记相关信息, 包括细胞来源、操作项目、操作时间、操作人等. 小心将培养瓶放入37℃, 5%CO<sub>2</sub>的培养箱中培养.</p>
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</td>
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</tr><tr class="sop-row-hidden sop-row-hidden">
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<td colspan="7" style="border:1px solid #334155; padding:8px;">每个培养瓶上皆清楚标示细胞批号</td>
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<td colspan="5" class="sop-option-cell" style="border:1px solid #334155; padding:8px; text-align:center;">
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<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>是</span>
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<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>否</span>
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</td>
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</tr><tr class="sop-row-hidden sop-row-hidden">
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<td rowspan="3" style="border:1px solid #334155; padding:8px; text-align:center; vertical-align:middle;">清场</td>
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<td colspan="6" style="border:1px solid #334155; padding:8px;">未使用完试剂是否放入指定位置</td>
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<td colspan="5" class="sop-option-cell" style="border:1px solid #334155; padding:8px; text-align:center;">
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<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>是</span>
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<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>否</span>
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</td>
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</tr><tr class="sop-row-hidden sop-row-hidden">
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<td colspan="6" style="border:1px solid #334155; padding:8px;">废液是否进行收集处理</td>
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<td colspan="5" class="sop-option-cell" style="border:1px solid #334155; padding:8px; text-align:center;">
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<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>是</span>
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<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>否</span>
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</td>
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</tr><tr class="sop-row-hidden sop-row-hidden">
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<td colspan="4" style="border:1px solid #334155; padding:8px; white-space:nowrap;">是否清洁, 消毒操作间</td>
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<td colspan="1" class="sop-option-cell" style="border:1px solid #334155; padding:8px; white-space:nowrap; text-align:center;">
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<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>是</span>
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<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>否</span>
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</td>
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<td colspan="5" style="border:1px solid #334155; padding:8px; white-space:nowrap;">是否清洁, 消毒生物安全柜与移液枪</td>
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<td colspan="1" class="sop-option-cell" style="border:1px solid #334155; padding:8px; white-space:nowrap; text-align:center;">
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<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>是</span>
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<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>否</span>
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</td>
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</tr><tr class="sop-row-hidden sop-row-hidden">
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<td style="border:1px solid #334155; padding:8px; text-align:center;">备注</td>
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<td colspan="11" style="border:1px solid #334155; padding:8px; min-height:48px; height:48px; vertical-align:top;"> </td>
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</tr><tr class="sop-row-hidden sop-row-hidden">
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<td colspan="4" style="border:1px solid #334155; padding:10px;">操作人/日期:</td>
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<td colspan="5" style="border:1px solid #334155; padding:10px;">复核人/日期:</td>
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<td colspan="3" style="border:1px solid #334155; padding:10px;">主管/日期:</td>
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</tr></tbody>
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</table>
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</div>
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',
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`update_time` = NOW()
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WHERE `process_code` = 'SOP-SC-003-E01-01'
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AND `del_flag` = 0;
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