328 lines
30 KiB
HTML
328 lines
30 KiB
HTML
<!-- SOP-SC-014-E01-01 NK细胞培养操作记录(约2页 A4 竖版) -->
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<div class="sop-record sop-a4 sop-a4-pages-1 sop-sc-014" style="font-family: 'PingFang SC','Microsoft YaHei',sans-serif; font-size: 10.5pt; color: #1e293b; line-height: 1.55; box-sizing: border-box; display: flex; flex-direction: column; height: 297mm; min-height: 297mm; padding: 16mm 15mm 20mm;">
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<table class="sop-header-table sop-row-hidden" style="width:100%; border-collapse:collapse; table-layout:fixed; border:1px solid #334155; margin-bottom:8px; flex-shrink:0;">
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<colgroup>
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<col style="width:15%" />
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<col style="width:48%" />
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<col style="width:37%" />
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</colgroup>
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<tbody>
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<tr>
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<td rowspan="2" style="border:1px solid #334155; padding:6px; text-align:center; vertical-align:middle;">
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<img src="/sop-templates/SOP-SC-014-logo.jpeg" alt="logo" width="48" height="48" style="display:block; margin:0 auto;" />
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</td>
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<td rowspan="2" style="border:1px solid #334155; padding:8px; text-align:center; vertical-align:middle; font-family: SimSun, 'Songti SC', serif; font-size: 12pt; font-weight:700;">
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NK细胞培养操作记录
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</td>
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<td style="border:1px solid #334155; padding:6px 10px; font-size: 12pt; vertical-align:middle;">
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文件编码: SOP-SC-014-E01
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</td>
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</tr>
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<tr>
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<td style="border:1px solid #334155; padding:6px 10px; font-size: 12pt; vertical-align:middle;">
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版 本 号: 01
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</td>
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</tr>
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</tbody>
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</table>
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<table class="sop-body-table" style="width:100%; border-collapse:collapse; table-layout:fixed; border:1px solid #334155; flex:0 1 auto; height:auto;">
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<colgroup>
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<col style="width:8.1%" />
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<col style="width:3.3%" />
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<col style="width:20.2%" />
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<col style="width:1.4%" />
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<col style="width:2.4%" />
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<col style="width:14.7%" />
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<col style="width:5.7%" />
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<col style="width:5.7%" />
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<col style="width:2.4%" />
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<col style="width:17.1%" />
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<col style="width:5.1%" />
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<col style="width:13.9%" />
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</colgroup>
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<tbody><tr class="sop-row-hidden sop-row-hidden">
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<td colspan="6" style="border:1px solid #334155; padding:8px;">工序: NK细胞培养</td>
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<td colspan="2" style="border:1px solid #334155; padding:8px; white-space:nowrap;">记录编号</td>
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<td colspan="4" class="sop-blank-cell" style="border:1px solid #334155; padding:8px; min-height:28px;"> </td>
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</tr><tr class="sop-row-hidden sop-row-hidden">
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<td colspan="2" style="border:1px solid #334155; padding:8px; white-space:nowrap;">细胞批号</td>
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<td colspan="4" class="sop-blank-cell" style="border:1px solid #334155; padding:8px;"> </td>
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<td colspan="2" style="border:1px solid #334155; padding:8px; white-space:nowrap;">日期</td>
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<td colspan="4" class="sop-blank-cell" style="border:1px solid #334155; padding:8px;"> </td>
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</tr><tr class="sop-row-hidden sop-row-hidden">
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<td rowspan="8" style="border:1px solid #334155; padding:3px 6px; text-align:center; vertical-align:middle; line-height:1.25;">试剂</td>
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<td colspan="2" style="border:1px solid #334155; padding:3px 6px; text-align:center; line-height:1.25;">名称</td>
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<td colspan="5" style="border:1px solid #334155; padding:3px 6px; text-align:center; line-height:1.25;">生产厂家</td>
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<td colspan="2" style="border:1px solid #334155; padding:3px 6px; text-align:center; line-height:1.25;">批号</td>
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<td colspan="2" style="border:1px solid #334155; padding:3px 6px; text-align:center; line-height:1.25;">使用数量</td>
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</tr><tr class="sop-row-hidden sop-row-hidden">
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<td colspan="2" style="border:1px solid #334155; padding:3px 6px; line-height:1.25;">UBMC/PBMC</td>
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<td colspan="5" style="border:1px solid #334155; padding:3px 6px; text-align:center; line-height:1.25;">/</td>
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<td colspan="2" class="sop-blank-cell" style="border:1px solid #334155; padding:3px 6px; line-height:1.25;"> </td>
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<td colspan="2" class="sop-blank-cell" style="border:1px solid #334155; padding:3px 6px; line-height:1.25;"> </td>
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</tr><tr class="sop-row-hidden sop-row-hidden">
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<td colspan="2" style="border:1px solid #334155; padding:3px 6px; line-height:1.25;">血替/血浆</td>
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<td colspan="5" style="border:1px solid #334155; padding:3px 6px; text-align:center; line-height:1.25;">/</td>
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<td colspan="2" class="sop-blank-cell" style="border:1px solid #334155; padding:3px 6px; line-height:1.25;"> </td>
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<td colspan="2" class="sop-blank-cell" style="border:1px solid #334155; padding:3px 6px; line-height:1.25;"> </td>
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</tr><tr class="sop-row-hidden sop-row-hidden">
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<td colspan="2" style="border:1px solid #334155; padding:3px 6px; line-height:1.25;">NK培养基1</td>
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<td colspan="5" style="border:1px solid #334155; padding:3px 6px; text-align:center; line-height:1.25;">/</td>
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<td colspan="2" class="sop-blank-cell" style="border:1px solid #334155; padding:3px 6px; line-height:1.25;"> </td>
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<td colspan="2" class="sop-blank-cell" style="border:1px solid #334155; padding:3px 6px; line-height:1.25;"> </td>
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</tr><tr class="sop-row-hidden sop-row-hidden">
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<td colspan="2" style="border:1px solid #334155; padding:3px 6px; line-height:1.25;">NK培养基2</td>
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<td colspan="5" style="border:1px solid #334155; padding:3px 6px; text-align:center; line-height:1.25;">/</td>
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<td colspan="2" class="sop-blank-cell" style="border:1px solid #334155; padding:3px 6px; line-height:1.25;"> </td>
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<td colspan="2" class="sop-blank-cell" style="border:1px solid #334155; padding:3px 6px; line-height:1.25;"> </td>
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</tr><tr class="sop-row-hidden sop-row-hidden">
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<td colspan="2" style="border:1px solid #334155; padding:3px 6px; line-height:1.25;">PBS</td>
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<td colspan="5" class="sop-blank-cell" style="border:1px solid #334155; padding:3px 6px; line-height:1.25;"> </td>
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<td colspan="2" class="sop-blank-cell" style="border:1px solid #334155; padding:3px 6px; line-height:1.25;"> </td>
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<td colspan="2" class="sop-blank-cell" style="border:1px solid #334155; padding:3px 6px; line-height:1.25;"> </td>
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</tr><tr class="sop-row-hidden sop-row-hidden">
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<td colspan="2" style="border:1px solid #334155; padding:3px 6px; line-height:1.25;">NK营养剂</td>
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<td colspan="5" style="border:1px solid #334155; padding:3px 6px; text-align:center; line-height:1.25;">/</td>
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<td colspan="2" class="sop-blank-cell" style="border:1px solid #334155; padding:3px 6px; line-height:1.25;"> </td>
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<td colspan="2" class="sop-blank-cell" style="border:1px solid #334155; padding:3px 6px; line-height:1.25;"> </td>
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</tr><tr class="sop-row-hidden sop-row-hidden">
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<td colspan="2" style="border:1px solid #334155; padding:3px 6px; line-height:1.25;">NK包被剂</td>
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<td colspan="5" style="border:1px solid #334155; padding:3px 6px; text-align:center; line-height:1.25;">/</td>
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<td colspan="2" class="sop-blank-cell" style="border:1px solid #334155; padding:3px 6px; line-height:1.25;"> </td>
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<td colspan="2" class="sop-blank-cell" style="border:1px solid #334155; padding:3px 6px; line-height:1.25;"> </td>
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</tr><tr class="sop-row-hidden sop-row-hidden">
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<td style="border:1px solid #334155; padding:3px 6px; text-align:center; vertical-align:middle; line-height:1.25;">仪器<br />设备</td>
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<td colspan="11" style="border:1px solid #334155; padding:3px 6px; vertical-align:middle; line-height:1.25;">
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<p style="margin:0;">
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生物安全柜:
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<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>XW015</span>
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<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>XW022</span>
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<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>其他</span>
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<span class="sop-underline" style="text-align:left; min-width:80px;"> </span>
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</p>
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<p style="margin:0;">
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离心机:
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<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>XW016</span>
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<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>XW026</span>
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<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>其他</span>
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<span class="sop-underline" style="text-align:left; min-width:80px;"> </span>
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</p>
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</td>
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</tr><tr class="sop-fill-row">
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<td class="sop-fill-label" style="border:1px solid #334155; padding:8px; text-align:center; vertical-align:middle; height:100%;">NK细胞<br />培养</td>
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<td class="sop-fill-cell" colspan="11" style="border:1px solid #334155; padding:10px; vertical-align:top; height:100%; word-wrap:break-word; overflow-wrap:break-word;">
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<p style="margin:0 0 6px;">
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1. 分离操作记录编号<span class="sop-underline" style="text-align:left; min-width:140px;"> </span>;
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冻存及复苏操作记录编号<span class="sop-underline" style="text-align:left; min-width:160px;"> </span>;
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</p>
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<p style="margin:0 0 6px;">2. 包被:</p>
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<p style="margin:0 0 6px; padding-left:4ch;">
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<span class="sop-option sop-option-wrap"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span><span class="sop-option-text">Day-1, 将3mL PBS与100μL NK包被剂混匀后加入T25培养瓶中, 平放晃匀. 铺满瓶底后置于4℃包被过夜;</span></span>
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</p>
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<p style="margin:0 0 6px; padding-left:4ch;">
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<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>37℃包被2h;</span>
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</p>
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<p style="margin:0 0 6px;">3. Day 0, 使用10mL PBS轻柔地清洗瓶底并吸出后待用;</p>
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<p style="margin:0 0 6px;">
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4. 接种<span class="sop-underline" style="text-align:left; min-width:64px;"> </span>*10<sup>7</sup>个细胞;
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</p>
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<p style="margin:0 0 6px;">5. 定容:</p>
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<p style="margin:0 0 6px; padding-left:4ch;">
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<span class="sop-option sop-option-wrap"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span><span class="sop-option-text">加入血替1mL之后, 用NK培养基1定容至10mL.</span></span>
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</p>
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<p style="margin:0 0 6px; padding-left:4ch;">
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<span class="sop-option sop-option-wrap"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span><span class="sop-option-text">加入自体血浆1mL之后, 用NK培养基1定容至10mL.</span></span>
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</p>
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<p style="margin:0 0 6px; padding-left:4ch;">做好标记, CO<sub>2</sub>培养箱中培养.;</p>
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<p style="margin:0 0 6px;">6. Day 3,</p>
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<p style="margin:0 0 6px; padding-left:4ch;">
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<span class="sop-option sop-option-wrap"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span><span class="sop-option-text">向T25培养瓶中加入血替1.5mL后向瓶中加入NK培养基1 13.5mL;</span></span>
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</p>
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<p style="margin:0 0 6px; padding-left:4ch;">
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<span class="sop-option sop-option-wrap"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span><span class="sop-option-text">向T25培养瓶中加入自体血浆1.5mL后向瓶中加入NK培养基1 13.5mL.</span></span>
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</p>
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<p style="margin:0 0 8px; padding-left:4ch;">轻晃混匀, 避免反复吹打, 做好标记, CO<sub>2</sub>培养箱中培养;</p>
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<p style="margin:0 0 6px;">7. 补液记录:</p>
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<table class="sop-grid-table" border="1" style="width:100%; border-collapse:collapse; table-layout:fixed; border:1px solid #334155; margin:6px 0;">
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<colgroup>
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<col style="width:11.0%" />
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<col style="width:10.5%" />
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<col style="width:9.0%" />
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<col style="width:10.5%" />
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<col style="width:14.0%" />
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<col style="width:12.5%" />
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<col style="width:14.5%" />
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<col style="width:18.0%" />
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</colgroup>
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<tbody>
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<tr>
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<td style="border:1px solid #334155; padding:4px 3px; text-align:center; font-size:9pt;">
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<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>PBMC</span>
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<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>UBMC</span>
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</td>
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<td style="border:1px solid #334155; padding:4px 3px; text-align:center; font-size:9pt;">NK培养基2<br />体积</td>
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<td style="border:1px solid #334155; padding:4px 3px; text-align:center; font-size:9pt;">NK营养剂<br />体积</td>
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<td style="border:1px solid #334155; padding:4px 3px; text-align:center; font-size:9pt; line-height:1.35;">
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<div style="margin:0 0 2px;">
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<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>血浆</span>
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</div>
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<div style="margin:0 0 2px;">
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<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>血替</span>
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</div>
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<div>体积</div>
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</td>
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<td style="border:1px solid #334155; padding:4px 3px; text-align:center; font-size:9pt;">补液前<br />计数结果</td>
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<td style="border:1px solid #334155; padding:4px 3px; text-align:center; font-size:9pt;">补液后<br />总体积</td>
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<td style="border:1px solid #334155; padding:4px 3px; text-align:center; font-size:9pt;">是否转瓶/袋</td>
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<td style="border:1px solid #334155; padding:4px 3px; text-align:center; font-size:9pt;">操作人/<br />复核人</td>
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</tr>
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<tr>
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<td style="border:1px solid #334155; padding:4px 3px; font-size:9pt; text-align:center; line-height:1.35;">Day<br /><span class="sop-underline" style="text-align:left; min-width:36px;"> </span></td>
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<td style="border:1px solid #334155; padding:4px 3px; font-size:9pt;"><span class="sop-underline" style="text-align:left; min-width:36px;"> </span>mL 90%</td>
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<td style="border:1px solid #334155; padding:4px 3px; font-size:9pt;"><span class="sop-underline" style="text-align:left; min-width:28px;"> </span>mL 5%</td>
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<td style="border:1px solid #334155; padding:4px 3px; font-size:9pt;"><span class="sop-underline" style="text-align:left; min-width:28px;"> </span>mL 5%</td>
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<td style="border:1px solid #334155; padding:4px 3px; font-size:9pt; text-align:center; line-height:1.35;"><span class="sop-underline" style="text-align:left; min-width:52px;"> </span>*10<sup>6</sup><br />/mL</td>
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<td style="border:1px solid #334155; padding:4px 3px; font-size:9pt;"><span class="sop-underline" style="text-align:left; min-width:48px;"> </span>mL</td>
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<td style="border:1px solid #334155; padding:4px 3px; font-size:9pt; text-align:center; line-height:1.45;">
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<div style="margin:0;">
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<span class="sop-option" style="margin-right:0;"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>转瓶</span>
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</div>
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<div style="margin:0;">
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/<span class="sop-option" style="margin-right:0;"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>转袋</span>
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</div>
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</td>
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<td class="sop-blank-cell" style="border:1px solid #334155; padding:4px 3px; font-size:9pt;"> </td>
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</tr>
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<tr>
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<td style="border:1px solid #334155; padding:4px 3px; font-size:9pt; text-align:center; line-height:1.35;">Day<br /><span class="sop-underline" style="text-align:left; min-width:36px;"> </span></td>
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<td style="border:1px solid #334155; padding:4px 3px; font-size:9pt;"><span class="sop-underline" style="text-align:left; min-width:36px;"> </span>mL 95%</td>
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<td style="border:1px solid #334155; padding:4px 3px; font-size:9pt;"><span class="sop-underline" style="text-align:left; min-width:28px;"> </span>mL 2.5%</td>
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<td style="border:1px solid #334155; padding:4px 3px; font-size:9pt;"><span class="sop-underline" style="text-align:left; min-width:28px;"> </span>mL 2.5%</td>
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<td style="border:1px solid #334155; padding:4px 3px; font-size:9pt; text-align:center; line-height:1.35;"><span class="sop-underline" style="text-align:left; min-width:52px;"> </span>*10<sup>6</sup><br />/mL</td>
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<td style="border:1px solid #334155; padding:4px 3px; font-size:9pt;"><span class="sop-underline" style="text-align:left; min-width:48px;"> </span>mL</td>
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<td style="border:1px solid #334155; padding:4px 3px; font-size:9pt; text-align:center; line-height:1.45;">
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<div style="margin:0;">
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<span class="sop-option" style="margin-right:0;"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>转瓶</span>
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</div>
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<div style="margin:0;">
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/<span class="sop-option" style="margin-right:0;"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>转袋</span>
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</div>
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</td>
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<td class="sop-blank-cell" style="border:1px solid #334155; padding:4px 3px; font-size:9pt;"> </td>
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</tr>
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<tr>
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<td style="border:1px solid #334155; padding:4px 3px; font-size:9pt; text-align:center; line-height:1.35;">Day<br /><span class="sop-underline" style="text-align:left; min-width:36px;"> </span></td>
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<td style="border:1px solid #334155; padding:4px 3px; font-size:9pt;"><span class="sop-underline" style="text-align:left; min-width:36px;"> </span>mL 99%</td>
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<td style="border:1px solid #334155; padding:4px 3px; font-size:9pt;"><span class="sop-underline" style="text-align:left; min-width:28px;"> </span>mL 1%</td>
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<td style="border:1px solid #334155; padding:4px 3px; font-size:9pt; text-align:center;">/</td>
|
|
<td style="border:1px solid #334155; padding:4px 3px; font-size:9pt; text-align:center; line-height:1.35;"><span class="sop-underline" style="text-align:left; min-width:52px;"> </span>*10<sup>6</sup><br />/mL</td>
|
|
<td style="border:1px solid #334155; padding:4px 3px; font-size:9pt;"><span class="sop-underline" style="text-align:left; min-width:48px;"> </span>mL</td>
|
|
<td style="border:1px solid #334155; padding:4px 3px; font-size:9pt; text-align:center; line-height:1.45;">
|
|
<div style="margin:0;">
|
|
<span class="sop-option" style="margin-right:0;"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>转瓶</span>
|
|
</div>
|
|
<div style="margin:0;">
|
|
/<span class="sop-option" style="margin-right:0;"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>转袋</span>
|
|
</div>
|
|
</td>
|
|
<td class="sop-blank-cell" style="border:1px solid #334155; padding:4px 3px; font-size:9pt;"> </td>
|
|
</tr>
|
|
<tr>
|
|
<td style="border:1px solid #334155; padding:4px 3px; font-size:9pt; text-align:center; line-height:1.35;">Day<br /><span class="sop-underline" style="text-align:left; min-width:36px;"> </span></td>
|
|
<td style="border:1px solid #334155; padding:4px 3px; font-size:9pt;"><span class="sop-underline" style="text-align:left; min-width:36px;"> </span>mL 99.5%</td>
|
|
<td style="border:1px solid #334155; padding:4px 3px; font-size:9pt;"><span class="sop-underline" style="text-align:left; min-width:28px;"> </span>mL 0.5%</td>
|
|
<td style="border:1px solid #334155; padding:4px 3px; font-size:9pt; text-align:center;">/</td>
|
|
<td style="border:1px solid #334155; padding:4px 3px; font-size:9pt; text-align:center; line-height:1.35;"><span class="sop-underline" style="text-align:left; min-width:52px;"> </span>*10<sup>6</sup><br />/mL</td>
|
|
<td style="border:1px solid #334155; padding:4px 3px; font-size:9pt;"><span class="sop-underline" style="text-align:left; min-width:48px;"> </span>mL</td>
|
|
<td style="border:1px solid #334155; padding:4px 3px; font-size:9pt; text-align:center; line-height:1.45;">
|
|
<div style="margin:0;">
|
|
<span class="sop-option" style="margin-right:0;"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>转瓶</span>
|
|
</div>
|
|
<div style="margin:0;">
|
|
/<span class="sop-option" style="margin-right:0;"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>转袋</span>
|
|
</div>
|
|
</td>
|
|
<td class="sop-blank-cell" style="border:1px solid #334155; padding:4px 3px; font-size:9pt;"> </td>
|
|
</tr>
|
|
<tr>
|
|
<td style="border:1px solid #334155; padding:4px 3px; font-size:9pt; text-align:center; line-height:1.35;">Day<br /><span class="sop-underline" style="text-align:left; min-width:36px;"> </span></td>
|
|
<td style="border:1px solid #334155; padding:4px 3px; font-size:9pt;"><span class="sop-underline" style="text-align:left; min-width:36px;"> </span>mL 99.5%</td>
|
|
<td style="border:1px solid #334155; padding:4px 3px; font-size:9pt;"><span class="sop-underline" style="text-align:left; min-width:28px;"> </span>mL 0.5%</td>
|
|
<td style="border:1px solid #334155; padding:4px 3px; font-size:9pt; text-align:center;">/</td>
|
|
<td style="border:1px solid #334155; padding:4px 3px; font-size:9pt; text-align:center; line-height:1.35;"><span class="sop-underline" style="text-align:left; min-width:52px;"> </span>*10<sup>6</sup><br />/mL</td>
|
|
<td style="border:1px solid #334155; padding:4px 3px; font-size:9pt;"><span class="sop-underline" style="text-align:left; min-width:48px;"> </span>mL</td>
|
|
<td style="border:1px solid #334155; padding:4px 3px; font-size:9pt; text-align:center; line-height:1.45;">
|
|
<div style="margin:0;">
|
|
<span class="sop-option" style="margin-right:0;"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>转瓶</span>
|
|
</div>
|
|
<div style="margin:0;">
|
|
/<span class="sop-option" style="margin-right:0;"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>转袋</span>
|
|
</div>
|
|
</td>
|
|
<td class="sop-blank-cell" style="border:1px solid #334155; padding:4px 3px; font-size:9pt;"> </td>
|
|
</tr>
|
|
<tr>
|
|
<td style="border:1px solid #334155; padding:4px 3px; font-size:9pt; text-align:center; line-height:1.35;">Day<br /><span class="sop-underline" style="text-align:left; min-width:36px;"> </span></td>
|
|
<td style="border:1px solid #334155; padding:4px 3px; font-size:9pt;"><span class="sop-underline" style="text-align:left; min-width:36px;"> </span>mL 99.5%</td>
|
|
<td style="border:1px solid #334155; padding:4px 3px; font-size:9pt;"><span class="sop-underline" style="text-align:left; min-width:28px;"> </span>mL 0.5%</td>
|
|
<td style="border:1px solid #334155; padding:4px 3px; font-size:9pt; text-align:center;">/</td>
|
|
<td style="border:1px solid #334155; padding:4px 3px; font-size:9pt; text-align:center; line-height:1.35;"><span class="sop-underline" style="text-align:left; min-width:52px;"> </span>*10<sup>6</sup><br />/mL</td>
|
|
<td style="border:1px solid #334155; padding:4px 3px; font-size:9pt;"><span class="sop-underline" style="text-align:left; min-width:48px;"> </span>mL</td>
|
|
<td style="border:1px solid #334155; padding:4px 3px; font-size:9pt; text-align:center;">/</td>
|
|
<td class="sop-blank-cell" style="border:1px solid #334155; padding:4px 3px; font-size:9pt;"> </td>
|
|
</tr>
|
|
<tr>
|
|
<td style="border:1px solid #334155; padding:4px 3px; font-size:9pt; text-align:center; line-height:1.35;">Day<br /><span class="sop-underline" style="text-align:left; min-width:36px;"> </span></td>
|
|
<td style="border:1px solid #334155; padding:4px 3px; font-size:9pt; text-align:center;">/</td>
|
|
<td style="border:1px solid #334155; padding:4px 3px; font-size:9pt; text-align:center;">/</td>
|
|
<td style="border:1px solid #334155; padding:4px 3px; font-size:9pt; text-align:center;">/</td>
|
|
<td style="border:1px solid #334155; padding:4px 3px; font-size:9pt; text-align:center;">收获细胞</td>
|
|
<td style="border:1px solid #334155; padding:4px 3px; font-size:9pt;"><span class="sop-underline" style="text-align:left; min-width:48px;"> </span>mL</td>
|
|
<td style="border:1px solid #334155; padding:4px 3px; font-size:9pt; text-align:center;">/</td>
|
|
<td class="sop-blank-cell" style="border:1px solid #334155; padding:4px 3px; font-size:9pt;"> </td>
|
|
</tr>
|
|
</tbody>
|
|
</table>
|
|
<p style="margin:8px 0 0;">
|
|
共计收获<span class="sop-underline" style="text-align:left; min-width:64px;"> </span>*10<sup>9</sup>个细胞, 活率:
|
|
<span class="sop-underline" style="text-align:left; min-width:56px;"> </span>; 直径:
|
|
<span class="sop-underline" style="text-align:left; min-width:56px;"> </span>μm.
|
|
</p>
|
|
<p style="margin:0; line-height:1.55;"> </p>
|
|
</td>
|
|
</tr><tr class="sop-row-hidden sop-row-hidden">
|
|
<td rowspan="3" style="border:1px solid #334155; padding:8px; text-align:center; vertical-align:middle;">清场</td>
|
|
<td colspan="6" style="border:1px solid #334155; padding:8px;">未使用完试剂是否放入指定位置</td>
|
|
<td colspan="5" class="sop-option-cell" style="border:1px solid #334155; padding:8px; text-align:center;">
|
|
<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>是</span>
|
|
<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>否</span>
|
|
</td>
|
|
</tr><tr class="sop-row-hidden sop-row-hidden">
|
|
<td colspan="6" style="border:1px solid #334155; padding:8px;">废液是否进行收集处理</td>
|
|
<td colspan="5" class="sop-option-cell" style="border:1px solid #334155; padding:8px; text-align:center;">
|
|
<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>是</span>
|
|
<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>否</span>
|
|
</td>
|
|
</tr><tr class="sop-row-hidden sop-row-hidden">
|
|
<td colspan="4" style="border:1px solid #334155; padding:8px; white-space:nowrap;">是否清洁, 消毒操作间</td>
|
|
<td colspan="1" class="sop-option-cell" style="border:1px solid #334155; padding:8px; white-space:nowrap; text-align:center;">
|
|
<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>是</span>
|
|
<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>否</span>
|
|
</td>
|
|
<td colspan="5" style="border:1px solid #334155; padding:8px; white-space:nowrap;">是否清洁, 消毒生物安全柜与移液枪</td>
|
|
<td colspan="1" class="sop-option-cell" style="border:1px solid #334155; padding:8px; white-space:nowrap; text-align:center;">
|
|
<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>是</span>
|
|
<span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false"> </span>否</span>
|
|
</td>
|
|
</tr><tr class="sop-row-hidden sop-row-hidden">
|
|
<td style="border:1px solid #334155; padding:8px; text-align:center;">备注</td>
|
|
<td colspan="11" style="border:1px solid #334155; padding:8px; min-height:48px; height:48px; vertical-align:top;"> </td>
|
|
</tr><tr class="sop-row-hidden sop-row-hidden">
|
|
<td colspan="4" style="border:1px solid #334155; padding:10px;">操作人/日期:</td>
|
|
<td colspan="5" style="border:1px solid #334155; padding:10px;">复核人/日期:</td>
|
|
<td colspan="3" style="border:1px solid #334155; padding:10px;">主管/日期:</td>
|
|
</tr></tbody>
|
|
</table>
|
|
</div>
|